Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. sagesteele @P_McCulloughMD While I dont know whether there is a connection between Tylenol and autism, I do believe that there is reason to STOP giving it to babies children. Glutathione is impacted by Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: Acetaminophen Depletes Glutathione Essential Formulas