Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Early life acetaminophen exposure, glutathione S transferase genes, and development of adolescent asthma in a high risk birth cohort Journal of Allergy and Clinical Immunology Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Oxidative stress mediates end organ damage in a novel model of acetaminophen toxicity in Drosophila Scientific Reports