Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Circulating acetaminophen metabolites are toxicokinetic biomarkers of acute liver injury Vliegenthart 2017 Clinical Pharmacology & Therapeutics Wiley Online Library Full article: Paracetamol (acetaminophen) overdose and hepatotoxicity: mechanism, treatment, prevention measures, and estimates of burden of disease Acetaminophen, ethanol and other co ingestions ScienceDirect
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does acetaminophen decrease glutathione Tylenol's Impact on Glutathione and Liver Damage