Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Jenny McCarthy's Glutathione and Tylenol Claims: Medical Toxicologist Weighs In MedPage Today Tylenol depletes glutathione your liver's most important protective antioxidant. Every dose chips away at it. So instead of taking lower doses every 46 hours, I recommend spacing to 1000mg every 8 hours. Benefits of Glutathione