Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. How Dangerous is Tylenol? USMLE Step 2 CK Question of the Day: Altered Mental State After Over the counter pain reliever and Prescription Hydrocodone acetaminophen Osmosis Blog Acetaminophen, also known as Tylenol, is used to help with pain & to reduce fever. However, taking too much acetaminophen can be harmful to the liver which filters out toxins &