Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Ferroptosis and Intrinsic Drug induced Liver Injury by Acetaminophen and Other Drugs: A Critical Evaluation and Historical Perspective Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: Tylenol's Impact on Glutathione and Liver Damage