Acetaminophen metabolism. Acetaminophen is metabolized primarily by Download Scientific Diagram Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Oxidative stress during acetaminophen hepatotoxicity: Sources, pathophysiological role and therapeutic potential ScienceDirect Pharmacokinetic profile of metabolites by heart cutting two dimensional liquid chromatography: A focus on paracetamol analysis ScienceDirect