Identification of cytotoxic, glutathione reactive moieties inducing accumulation of reactive oxygen species via glutathione depletion ScienceDirect Initial Glutathione Depletion During Short Term Bed Rest: Pinpointing Synthesis and Degradation Checkpoints in the Glutamyl Cycle Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. What to Avoid When Taking Glutathione Thornhill's Pharmacy