Since Tylenol is such a hot topic right now, did you know it depletes glutathione levels? When your body processes Tylenol in the liver, one of the pathways creates a toxic byproduct. To neutralize Taking Tylenol? The Glutathione Connection Dr. Green Mom Tylenol's Impact on Glutathione and Liver Damage Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.