Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome Scientific Reports Tylenol's Impact on Glutathione and Liver Damage Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Current Insights into Glutathione Depletion in Adult Septic Patients